| 质粒名称: | pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 163059 |
| 存储实验室: | Adam Cohen |
|
载体骨架基本信息 |
|
| 载体名称: | pLX304 |
| 载体出品公司: | -- |
| 空载体大小: | 9377 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,慢病毒载体 |
| 筛选标记: | 杀稻瘟菌素 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | mito-roGFP2-Orp1 |
| 物种来源: | Synthetic |
| 插入大小: | 1410 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | CMV-F |
| 3’测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial) was a gift from Adam Cohen (Addgene plasmid # 163059 ; http://n2t.net/addgene:163059 ; RRID:Addgene_163059) |
| 参考文献部分: | pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial) was a gift from Adam Cohen (Addgene plasmid # 163059 ; http://n2t.net/addgene:163059 ; RRID:Addgene_163059)Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE. Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5. 10.1038/s41467-020-17607-5 PubMed 32753572 |
pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondri
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pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondri载体质粒基本信息
pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondri质粒图谱载体图谱和pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondri载体序列质粒序列多克隆位点信息
质粒图谱.png)
pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondri质粒载体简介
pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondri质粒序列载体序列
LOCUS pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial) 9175 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial).
ACCESSION .
VERSION .
KEYWORDS pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9175)
AUTHORS Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
TITLE Multiplexed Optical Sensors in Arrayed Islands of Cells for
multimodal recordings of cellular physiology.
JOURNAL Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
PUBMED 32753572
REFERENCE 2 (bases 1 to 9175)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9175
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..181
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 228..353
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 846..1079
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1264..1308
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 1483..1983
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1902..1920
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 1995..2390
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 2450..2567
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2684..2987
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2988..3191
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3142..3162
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 3214..3238
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 3277..3351
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
CDS 3376..4089
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(3418..3439)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3679..3698)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4026..4047
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind complement(4692..4716)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 4718..4759
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
misc_feature 4801..5389
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4854..4874)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5272..5283)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5461..5694
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5772..5893
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5809..5828)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5863..5882
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5933..6068
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5995..6014
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6088..6107)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6089..6107)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6117..6134)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6117..6133)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6126..6148)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6275..6730
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6362..6381)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6572..6593
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6756..6860
/gene="bla"
/label=AmpR promoter
CDS 6861..7721
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7079..7098)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7892..8480
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8381..8400
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8634..8651
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 8768..8789
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8804..8834
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8842..8858
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8847..8869
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8866..8882
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8866..8882
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 8901..8921
/label=T3
/note="T3 promoter, forward primer"
promoter 8903..8921
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 8949..9175
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
ORIGIN
1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc
1501 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
1561 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
1621 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
1681 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
1741 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
1801 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
1861 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
1921 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
1981 cagggggtac caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
2041 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
2101 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
2161 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
2221 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
2281 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
2341 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc
2401 agtagtaaga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
2461 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
2521 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
2581 gggacagcag agatccactt tggcgccggc tcgagggggc ccggggttat taatagtaat
2641 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
2701 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
2761 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
2821 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
2881 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
2941 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
3001 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
3061 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
3121 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
3181 taagcagagc tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agcaggcttt
3241 aaaggaacca attcagtcga ctggatctgc ggccgcatgt ccgtcctgac gccgctgctg
3301 ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg
3361 ttgccgccgg gatccgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
3421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
3481 gatgccacct acggcaagct gaccctgaag ttcatctcca ccaccggcaa gctgcccgtg
3541 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
3601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
3661 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
3721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
3781 atcctggggc acaagctgga gtacaactac aactgccaca acgtctatat catggccgac
3841 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
3901 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
3961 cccgacaacc actacctgag cacctgctcc gccctgagca aagaccccaa cgagaagcgc
4021 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
4081 ctgtacaaga ctagtggtgg ttcaggtggt ggtggttcag gtggtggtgg ttcaggtgga
4141 ggaggatcag gaggaggagg atcaggagga ggaggatcag gaggagaatt cgatatctca
4201 gaattctata agctagcacc tgttgacaag aaaggccaac cattcccctt cgaccaatta
4261 aagggaaaag tggtgcttat cgttaatgtt gcctccaaat gtggattcac tcctcaatac
4321 aaagaactag aggccttgta caaacgttat aaggacgaag gatttaccat catcgggttc
4381 ccatgcaacc agtttggcca ccaagaacct ggctctgatg aagaaattgc ccagttctgc
4441 caactgaact atggcgtgac tttccccatt atgaaaaaaa ttgacgttaa tggtggcaat
4501 gaggaccctg tttacaagtt tttgaagagc caaaaatccg gtatgttggg cttgagaggt
4561 atcaaatgga attttgaaaa attcttagtc gataaaaagg gtaaagtgta cgaaagatac
4621 tcttcactaa ccaaaccttc ttcgttgtcc gaaaccatcg aagaactttt gaaagaggtg
4681 gaatagtcta gacccagctt tcttgtacaa agtggttggt aagcctatcc ctaaccctct
4741 cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac
4801 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
4861 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
4921 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
4981 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
5041 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
5101 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
5161 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
5221 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
5281 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
5341 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
5401 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
5461 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
5521 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
5581 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
5641 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
5701 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
5761 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
5821 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
5881 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
5941 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
6001 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
6061 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
6121 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
6181 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
6241 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
6301 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
6361 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
6421 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
6481 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
6541 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
6601 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
6661 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
6721 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
6781 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
6841 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
6901 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
6961 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
7021 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
7081 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
7141 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
7201 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
7261 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
7321 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
7381 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
7441 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
7501 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
7561 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
7621 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
7681 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
7741 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
7801 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
7861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
7921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
7981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
8041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
8101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
8161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
8221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
8281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
8341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
8401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
8461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
8521 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
8581 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
8641 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
8701 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
8761 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
8821 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
8881 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
8941 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
9001 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
9061 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
9121 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaac
//
